Biological Consulting Services of North Central Florida, Inc.

Your source for accurate biological testing.

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Biosolids, Class A, Sludge, & Wastewater

Biosolids Testing Laboratory Utilizing EPA Principal Biosolids Guidance; EPA/625/R-92/013

Our laboratory team routinely monitors for various human pathogens in biosolids and soil.  We typically analyze for enteric viruses, Cryptosporidium and Giardia, helminth ova, and bacterial pathogens. We are currently involved in numerous pathogen monitoring projects of biosolids and biosolid amended soils within the state of Florida, throughout the United States, and internationally.

EPA Part 503 Biosolids Rule

This compre­hensive, risk-based rule was first published in the Federal Register on February 19, 1993. It was enacted to protect public health and the environment from reasonably anticipated adverse effects of pollutants that may be present in biosolids that are used or disposed. Much of the rule was based on the results of risk assess­ments that were scientifically conducted to identify what, if any, risks were associated with the use or disposal of biosolids.

Biosolids are the primarily organic solid product yielded by municipal wastewater treatment processes. Application of treated biosolids to land is a beneficial crop nutrient, soil conditioner and recyclable resource. However, if proper treatment and use criteria are not met, there is a risk of releasing pathogens into the environment.

Subpart D of the Part 503 regulation defines the requirement for biosolids to be classified as either Class A or B with respect to pathogens. The classification of biosolids prior to land application provides a means to protect public health and the environment.

There are different rules for different classes of biosolids

Class A biosolids are highly treated and of exceptional quality. They contain negligible levels of pathogens and metals and meet strict vector attraction reduction requirements

Class B biosolids are treated, but still contain detectible levels of pathogens. There are buffer requirements, public access, and crop harvesting restrictions for virtually all forms of Class B biosolids

Routine monitoring of biosolids is essential to ensure quality requirements and public safety.

Environmental Regulations and Technology: Control of Pathogens and Vector Attraction in Sewage Sludge (PDF) is a publication that provides useful guidance for successful compliance with 40 CFR Part 503 Subpart D. This subpart is the regulatory backbone of Class A and Class B pathogen requirements discussed in this Web site as well as the foundation for the Pathogen Equivalency Committee. EPA/625/R-92/013 Principal Biosolids Guidance is a primary reference for regional, state, and local regulatory authorities and their constituents.


Method for the Recovery and Assay of Total Culturable Viruses from Sludge EPA/625/R-92/013 appendix H or Standard Practice for the Recovery of Viruses from Wastewater Sludges; ASTM D4994-89: Elution, concentration and viable enterovirus enumeration of sample (biosolids) by cell culture analysis on Buffalo Green Monkey cells, Rhabdosarcoma cells, and MA 104 cells.

$ Please call for pricing

Helminth Ova Detection and Viability Analysis: Test Method for Detecting, Enumerating, and Determining the Viability of Ascaris Ova in Sludge (PDF)
$ Please call for pricing

Fecal Coliform Analysis EPA 1681: MPN method for analysis of solids / soils

$ Please call for pricing

Salmonella in Sewage Sludge (Biosolids) Method 1682: MPN / Modified Semisolid Rappaport-Vassiliadis (MSRV) Medium $ Please Call for Pricing
Detection of coliphages in solids and biosolids $ Please Call for Pricing
Fecal Strep Enumeration by  Standard Method SM 9230B $ Please Call for Pricing
Total Coliform Enumeration by Standard Method MPN SM 9221B $ Please call for Pricing