Biological Consulting Services of North Central Florida, Inc.

Your source for accurate biological testing.

  • Increase font size
  • Default font size
  • Decrease font size

EPA 1623.1 Cryptosporidium and Giardia Analysis and Testing Laboratory


EPA 1623.1:  Cryptosporidium and Giardia in Water by Filtration/IMS/FA


Cryptosporidiosis is a intestinal disease caused by microscopic parasites of the genus Cryptosporidium. It causes severe diarrhea and is typically self limiting. Both the disease and the parasite are commonly referred to as "crypto" (CDC info page)

Many species of Cryptosporidium infect humans and a wide range of animals. The parasite is protected by an outer shell that allows it to survive outside the body for long periods of time and makes it very resistant to chlorine disinfection (EPA Advisory).

While this parasite can be transmitted in several different ways, water is a common method and Cryptosporidium is one of the most frequent causes of waterborne disease (drinking water and recreational water) among humans in the United States.


Giardiasis is an intestinal infection caused by the protozoan Giardia lamblia. Symptoms of infection are diarrhea, abdominal cramps, pale and greasy stools, fatigue, bloating, and weight loss. Diarrhea may last for days or weeks, or may come and go. Symptoms vary from person to person. Some people with giardiasis have no symptoms at all. Symptoms usually arise 5 to 25 days after swallowing the cyst. People and animals infected with giardiasis pass the parasite in their stool. People become infected either by direct hand-to-mouth transfer from the stool of an infected person or indirectly by swallowing water that has been contaminated with stool that contains Giardia (when swimming in ponds, drinking from streams, etc.).

Our laboratory is completely equipped for environmental parasitology and possesses all the equipment necessary for performing EPA Method 1623.1 for analysis of Cryptosporidium and Giardia.

Cryptosporidium and Giardia One Page Sampling Summary (pdf)

Method EPA 1623 and updated EPA 1623.1 Cryptosporidium and Giardia analysis


Cryptosporidium and Giardia Detection and Enumeration (EPA 1623 and Method EPA 1623.1) and LT-2. Price includes one Envirocheck HV Filter.  Laboratory staff will perform field sampling of up to 100 Liters using approved filter or accept a water sample that is shipped directly to the laboratory; typically 10 liters. Price does not include travel time or shipping charges. Should a client prefer to use own filters then please subtract $100 from this price. $ Please Call for Current and Contract Pricing
Envirocheck HV Filter; for analysis of water samples.  This price is the cost per  additional filter and does not include price of analysis or sample collection.  The standard test price includes one filter. $ Please call for Current and contract Pricing
Sampling Kit; includes insulated cooler, cooler packs, tubing, flow regulator, connections, and flow meter. $ No Charge
Matrix Spike analysis (Required initially and every 20 samples thereafter) $ Please Call for Pricing
Additional filters and analysis of collected sample (in case of highly turbid source waters that cause clogging) Max 1 per sampling event $ Call for Pricing
Analysis of additional subsamples as per method requirement (max 3 per sampling event).  This occurs if pellet volumes exceed 0.5 ml as is the case in highly turbid systems $ No Charge
Cryptosporidium Viability Testing: Concentrated samples from Method 1623 are inoculated onto HCT-8 cell monolayers in 8-well chamber glass cell culture slides and are incubated in a 5% CO2 atmosphere at 37° C for 48 hours. Viable Cryptosporidium oocysts are enumerated by the Foci Detection-Most Probable Number Method described by Slifko et al. (1999). Results are reported as viable oocysts per 100L.  
Helminth Ova Detection; Test Method for Detecting, Enumerating, and Determining the Viability of Ascaris Ova in Sludge (PDF) $ Please call for Pricing
Sample collection, apparatus set-up, transportation and travel charge Call for quotation.