Biological Consulting Services of North Central Florida, Inc.

Your source for accurate biological testing.

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Helminth Ova Testing Laboratory


Helminth ova are the infectious eggs of parasitic worms and can pose a risk to human health. They are secreted in the feces of infected individuals and become concentrated in sewage sludge. They are resistant to environmental conditions therefore State and Federal rules require the testing of biosolids for helminth ova and enteric viruses before land application. The Method used  for their detection is described at the EPA's website

Test Method for Detecting, Enumerating, and determining the Viability of Ascaris Ova in Sludge. EPA/625/R92/013

The utilized method is described in the EPA's publication entitled "Environmental Regulations and Technology, Control of Pathogens and Vector Attraction in Sewage Sludge (EPA/625/R-92/013) July 2003".  The document is a primary reference for regional, state, and local regulatory authorities and their constituents. It describes the federal requirements concerning pathogens in sewage sludge and septage destined for land application or surface disposal and guidance for meeting those requirements. The document contains minor revisions from the 1999 edition, including minor editorial changes, an updated list of EPA and state biosolids coordinators, and minor updates to the procedures for analyzing bacteria, helminth ova, and enteroviruses.


We also offer various modifications of this method to allow for the detection of these pathogens in sewer water, foods, and soil samples. Our analysts are trained and qualified to determine  the presence of even a small number of helminth ova in a sample. We also provide enumeration and viability testing for inactivation and filtration testing.


Helminth ova Detection and Viability Analysis

$ please call for pricing